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moryella indoligenest ccug 52648 dq377947 stomatobaculum longumt dsm 24645  (ATCC)
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ATCC moryella indoligenest ccug 52648 dq377947 stomatobaculum longumt dsm 24645
Moryella Indoligenest Ccug 52648 Dq377947 Stomatobaculum Longumt Dsm 24645, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s longum  (DSMZ)
94
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S Longum, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stomatobaculum longum  (DSMZ)
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DSMZ stomatobaculum longum
Figure 1 <t>Stomatobaculum</t> longum induces the progression of OSCC. (A) Bacterial clones of tumor and normal tissues after cultivation in both anaerobic and aerobic conditions with blood agar plate. (B) Heatmap of the top 25 differentially abundant taxa using 16S rRNA gene sequencing in tumor tissues. (C) Multiple testing correction of the differentially expressed bacterium between tumor and normal tissues. CI calculated by the bootstrap method using 95% CI. (D) Wilcoxon rank-sum test of S. longum in tumor and normal tissues. (E) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by CCK8 assay. (F) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by clone formation assay. (G) The expression of S. longum in normal, OSCC tissues, and peri- tumors by qPCR assay. Data are shown as mean ± SD. One-way ANOVA and Two-way ANOVA were used for statistical analysis; *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.
Stomatobaculum Longum, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n terminus  (Addgene inc)
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Addgene inc n terminus
Figure 1 <t>Stomatobaculum</t> longum induces the progression of OSCC. (A) Bacterial clones of tumor and normal tissues after cultivation in both anaerobic and aerobic conditions with blood agar plate. (B) Heatmap of the top 25 differentially abundant taxa using 16S rRNA gene sequencing in tumor tissues. (C) Multiple testing correction of the differentially expressed bacterium between tumor and normal tissues. CI calculated by the bootstrap method using 95% CI. (D) Wilcoxon rank-sum test of S. longum in tumor and normal tissues. (E) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by CCK8 assay. (F) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by clone formation assay. (G) The expression of S. longum in normal, OSCC tissues, and peri- tumors by qPCR assay. Data are shown as mean ± SD. One-way ANOVA and Two-way ANOVA were used for statistical analysis; *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.
N Terminus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcs2 3xflagprickle2 addgene  (Addgene inc)
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Addgene inc pcs2 3xflagprickle2 addgene
Figure 1 <t>Stomatobaculum</t> longum induces the progression of OSCC. (A) Bacterial clones of tumor and normal tissues after cultivation in both anaerobic and aerobic conditions with blood agar plate. (B) Heatmap of the top 25 differentially abundant taxa using 16S rRNA gene sequencing in tumor tissues. (C) Multiple testing correction of the differentially expressed bacterium between tumor and normal tissues. CI calculated by the bootstrap method using 95% CI. (D) Wilcoxon rank-sum test of S. longum in tumor and normal tissues. (E) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by CCK8 assay. (F) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by clone formation assay. (G) The expression of S. longum in normal, OSCC tissues, and peri- tumors by qPCR assay. Data are shown as mean ± SD. One-way ANOVA and Two-way ANOVA were used for statistical analysis; *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.
Pcs2 3xflagprickle2 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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candida glabrata 1 nd4l  (ATCC)
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ATCC candida glabrata 1 nd4l
Figure 1 <t>Stomatobaculum</t> longum induces the progression of OSCC. (A) Bacterial clones of tumor and normal tissues after cultivation in both anaerobic and aerobic conditions with blood agar plate. (B) Heatmap of the top 25 differentially abundant taxa using 16S rRNA gene sequencing in tumor tissues. (C) Multiple testing correction of the differentially expressed bacterium between tumor and normal tissues. CI calculated by the bootstrap method using 95% CI. (D) Wilcoxon rank-sum test of S. longum in tumor and normal tissues. (E) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by CCK8 assay. (F) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by clone formation assay. (G) The expression of S. longum in normal, OSCC tissues, and peri- tumors by qPCR assay. Data are shown as mean ± SD. One-way ANOVA and Two-way ANOVA were used for statistical analysis; *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.
Candida Glabrata 1 Nd4l, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dsm 24645 t  (ATCC)
90
ATCC dsm 24645 t
Figure 1 <t>Stomatobaculum</t> longum induces the progression of OSCC. (A) Bacterial clones of tumor and normal tissues after cultivation in both anaerobic and aerobic conditions with blood agar plate. (B) Heatmap of the top 25 differentially abundant taxa using 16S rRNA gene sequencing in tumor tissues. (C) Multiple testing correction of the differentially expressed bacterium between tumor and normal tissues. CI calculated by the bootstrap method using 95% CI. (D) Wilcoxon rank-sum test of S. longum in tumor and normal tissues. (E) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by CCK8 assay. (F) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by clone formation assay. (G) The expression of S. longum in normal, OSCC tissues, and peri- tumors by qPCR assay. Data are shown as mean ± SD. One-way ANOVA and Two-way ANOVA were used for statistical analysis; *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.
Dsm 24645 T, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dsm 24645 t  (BEI Resources)
90
BEI Resources dsm 24645 t
Figure 1 <t>Stomatobaculum</t> longum induces the progression of OSCC. (A) Bacterial clones of tumor and normal tissues after cultivation in both anaerobic and aerobic conditions with blood agar plate. (B) Heatmap of the top 25 differentially abundant taxa using 16S rRNA gene sequencing in tumor tissues. (C) Multiple testing correction of the differentially expressed bacterium between tumor and normal tissues. CI calculated by the bootstrap method using 95% CI. (D) Wilcoxon rank-sum test of S. longum in tumor and normal tissues. (E) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by CCK8 assay. (F) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by clone formation assay. (G) The expression of S. longum in normal, OSCC tissues, and peri- tumors by qPCR assay. Data are shown as mean ± SD. One-way ANOVA and Two-way ANOVA were used for statistical analysis; *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.
Dsm 24645 T, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1 Stomatobaculum longum induces the progression of OSCC. (A) Bacterial clones of tumor and normal tissues after cultivation in both anaerobic and aerobic conditions with blood agar plate. (B) Heatmap of the top 25 differentially abundant taxa using 16S rRNA gene sequencing in tumor tissues. (C) Multiple testing correction of the differentially expressed bacterium between tumor and normal tissues. CI calculated by the bootstrap method using 95% CI. (D) Wilcoxon rank-sum test of S. longum in tumor and normal tissues. (E) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by CCK8 assay. (F) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by clone formation assay. (G) The expression of S. longum in normal, OSCC tissues, and peri- tumors by qPCR assay. Data are shown as mean ± SD. One-way ANOVA and Two-way ANOVA were used for statistical analysis; *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.

Journal: International Journal of Nanomedicine

Article Title: Stomatobaculum longum-Derived Extracellular Vesicles Enhance Oral Squamous Cell Carcinoma Malignancy Through BRCA1/EXO1/TP53BP1 Modulation

doi: 10.2147/ijn.s491473

Figure Lengend Snippet: Figure 1 Stomatobaculum longum induces the progression of OSCC. (A) Bacterial clones of tumor and normal tissues after cultivation in both anaerobic and aerobic conditions with blood agar plate. (B) Heatmap of the top 25 differentially abundant taxa using 16S rRNA gene sequencing in tumor tissues. (C) Multiple testing correction of the differentially expressed bacterium between tumor and normal tissues. CI calculated by the bootstrap method using 95% CI. (D) Wilcoxon rank-sum test of S. longum in tumor and normal tissues. (E) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by CCK8 assay. (F) The proliferation of CAL27 cells co-cultured with S. longum, E. coli, L. rhamnosus, and F. nucleatum by clone formation assay. (G) The expression of S. longum in normal, OSCC tissues, and peri- tumors by qPCR assay. Data are shown as mean ± SD. One-way ANOVA and Two-way ANOVA were used for statistical analysis; *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.

Article Snippet: Stomatobaculum longum-Derived Extracellular Vesicles (SBL-EVs) Isolation S. longum (DSM 24645, purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ) by the Guangdong Institute of Microbiology, Guangzhou, Guangdong, P.R.

Techniques: Clone Assay, Sequencing, Cell Culture, CCK-8 Assay, Tube Formation Assay, Expressing

Figure 2 Stomatobaculum longum promote the progression of OSCC via extracellular vesicles. (A) The cell viability of CAL27 and SCC9 cells cultured with supernatants from S. longum supplemented with GW4869 or not was measured by CCK8 assay. (B) The cell clone formation of CAL27 and SCC9 cells cultured with supernatants from S. longum supplemented with GW4869 or not was detected by clone formation assay. (C) The cell proliferation of CAL27 and SCC9 cells cultured with supernatants from S. longum supplemented with GW4869 or not was analyzed by EDU staining. (D) The cell cycle analysis of CAL27 and SCC9 cells cultured with supernatants from S. longum supplemented with GW4869 or not was accessed by flow cytometry. (E) The tumor volume in nude mice injected with CAL27 and SCC9 cells cultured with supernatants from S. longum supplemented with GW4869 or not (n = 5 mice per group). Experiments were repeated at least three times. Data are shown as mean ± SD. Two-way ANOVA was used for statistical analysis; *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.

Journal: International Journal of Nanomedicine

Article Title: Stomatobaculum longum-Derived Extracellular Vesicles Enhance Oral Squamous Cell Carcinoma Malignancy Through BRCA1/EXO1/TP53BP1 Modulation

doi: 10.2147/ijn.s491473

Figure Lengend Snippet: Figure 2 Stomatobaculum longum promote the progression of OSCC via extracellular vesicles. (A) The cell viability of CAL27 and SCC9 cells cultured with supernatants from S. longum supplemented with GW4869 or not was measured by CCK8 assay. (B) The cell clone formation of CAL27 and SCC9 cells cultured with supernatants from S. longum supplemented with GW4869 or not was detected by clone formation assay. (C) The cell proliferation of CAL27 and SCC9 cells cultured with supernatants from S. longum supplemented with GW4869 or not was analyzed by EDU staining. (D) The cell cycle analysis of CAL27 and SCC9 cells cultured with supernatants from S. longum supplemented with GW4869 or not was accessed by flow cytometry. (E) The tumor volume in nude mice injected with CAL27 and SCC9 cells cultured with supernatants from S. longum supplemented with GW4869 or not (n = 5 mice per group). Experiments were repeated at least three times. Data are shown as mean ± SD. Two-way ANOVA was used for statistical analysis; *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.

Article Snippet: Stomatobaculum longum-Derived Extracellular Vesicles (SBL-EVs) Isolation S. longum (DSM 24645, purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ) by the Guangdong Institute of Microbiology, Guangzhou, Guangdong, P.R.

Techniques: Cell Culture, CCK-8 Assay, Tube Formation Assay, Staining, Cell Cycle Assay, Flow Cytometry, Injection

Figure 3 Extracellular vesicles derived from Stomatobaculum longum isolation and characterization. (A) The structural morphology of extracellular vesicles derived from S. longum (SBL-EVs) by Cryo-TEM. (B) The size distribution of the SBL-EVs was measured by NTA assay. (C) RNA constituents of the SBL-EVs were detected by nucleic acid electrophoresis. (D) The protein components of the SBL-EVs were discerned using silver staining. (E) The small molecules of the SBL-EVs were elucidated by HPLC analysis. (F) The uptake of the SBL-EVs by CAL27 and SCC9 cells was measured by PHK26 staining. (G) The biodistribution of the SBL-EVs labeled with Dil in tumor-bearing mice by an IVIS Spectrum In vivo Imaging System. Experiments were repeated at least three times.

Journal: International Journal of Nanomedicine

Article Title: Stomatobaculum longum-Derived Extracellular Vesicles Enhance Oral Squamous Cell Carcinoma Malignancy Through BRCA1/EXO1/TP53BP1 Modulation

doi: 10.2147/ijn.s491473

Figure Lengend Snippet: Figure 3 Extracellular vesicles derived from Stomatobaculum longum isolation and characterization. (A) The structural morphology of extracellular vesicles derived from S. longum (SBL-EVs) by Cryo-TEM. (B) The size distribution of the SBL-EVs was measured by NTA assay. (C) RNA constituents of the SBL-EVs were detected by nucleic acid electrophoresis. (D) The protein components of the SBL-EVs were discerned using silver staining. (E) The small molecules of the SBL-EVs were elucidated by HPLC analysis. (F) The uptake of the SBL-EVs by CAL27 and SCC9 cells was measured by PHK26 staining. (G) The biodistribution of the SBL-EVs labeled with Dil in tumor-bearing mice by an IVIS Spectrum In vivo Imaging System. Experiments were repeated at least three times.

Article Snippet: Stomatobaculum longum-Derived Extracellular Vesicles (SBL-EVs) Isolation S. longum (DSM 24645, purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ) by the Guangdong Institute of Microbiology, Guangzhou, Guangdong, P.R.

Techniques: Derivative Assay, Isolation, Nucleic Acid Electrophoresis, Silver Staining, Staining, Labeling, In Vivo Imaging

Figure 4 Extracellular vesicles derived from Stomatobaculum longum promote the malignant phenotype of OSCC cells. (A) The cell viability of CAL27 and SCC9 cells cultured with SBL-EVs was measured by CCK8 assay. (B) The cell clone formation of CAL27 and SCC9 cells cultured with SBL-EVs was detected by clone formation assay. (C) The cell proliferation of CAL27 and SCC9 cells cultured with SBL-EVs was analyzed by EDU staining. (D) The cell cycle analysis of CAL27 and SCC9 cells cultured with SBL-EVs was accessed by flow cytometry. (E) The tumor volume in nude mice injected with CAL27 and SCC9 cells cultured with SBL-EVs (n = 5 mice per group). Experiments were repeated at least three times. Data are shown as mean ± SD. Two-way ANOVA was used for statistical analysis; **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.

Journal: International Journal of Nanomedicine

Article Title: Stomatobaculum longum-Derived Extracellular Vesicles Enhance Oral Squamous Cell Carcinoma Malignancy Through BRCA1/EXO1/TP53BP1 Modulation

doi: 10.2147/ijn.s491473

Figure Lengend Snippet: Figure 4 Extracellular vesicles derived from Stomatobaculum longum promote the malignant phenotype of OSCC cells. (A) The cell viability of CAL27 and SCC9 cells cultured with SBL-EVs was measured by CCK8 assay. (B) The cell clone formation of CAL27 and SCC9 cells cultured with SBL-EVs was detected by clone formation assay. (C) The cell proliferation of CAL27 and SCC9 cells cultured with SBL-EVs was analyzed by EDU staining. (D) The cell cycle analysis of CAL27 and SCC9 cells cultured with SBL-EVs was accessed by flow cytometry. (E) The tumor volume in nude mice injected with CAL27 and SCC9 cells cultured with SBL-EVs (n = 5 mice per group). Experiments were repeated at least three times. Data are shown as mean ± SD. Two-way ANOVA was used for statistical analysis; **p < 0.01, and ***p < 0.001. Abbreviation: ns, no significance.

Article Snippet: Stomatobaculum longum-Derived Extracellular Vesicles (SBL-EVs) Isolation S. longum (DSM 24645, purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ) by the Guangdong Institute of Microbiology, Guangzhou, Guangdong, P.R.

Techniques: Derivative Assay, Cell Culture, CCK-8 Assay, Tube Formation Assay, Staining, Cell Cycle Assay, Flow Cytometry, Injection